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                化工院:Synthesis of Polypeptides via Bioinspired Polymerization of in situ Purified N-carboxyanhydrides
                学术地点 新化工楼B 区106 报告厅 主讲人 Prof. Jianjun Cheng
                讲座时间 2019年6月25日 上午10:00

                主题:Synthesis of Polypeptides via Bioinspired Polymerization of in situ Purified N-carboxyanhydrides

                时间:2019年6月25日 上午10:00

                地点:新化工楼B 区106 报告厅

                主讲人:Prof. Jianjun Cheng, University of Illinois at U-C

                主讲人简介:

                Jianjun Cheng is the Hans Thurnauer Professor of Materials Science and Engineering, and Professor of Chemistry and Bioengineering at the University of Illinois at Urbana-Champaign (UIUC). He obtained a B.S. degree in Chemistry at Nankai University, China, in 1993, a M.S. degree in Chemistry at Southern Illinois University at Carbondale in 1996, and a Ph.D. degree in Materials Science at University of California, Santa Barbara in 2001. He was a Senior Scientist at Insert Therapeutics, Inc. from 2001 to 2004, and was a Postdoctoral Scientist at MIT from 2004 to 2005. He joined the faculty of UIUC as a tenure-track Assistant Professor in 2005, and was promoted to Associate Professor in 2011 and Full Professor in 2015. Prof. Cheng is the co-inventor of 40 patents and patent applications, and co-authored over 190 publications. He received National Science Foundation CAREER Award and NIH Director’s New Innovator Award. Cheng is currently an Associate Editor of Biomaterials Science, Royal Society of Chemistry. He is a Fellow of the American Institute for Medical and Biological Engineering, American Chemical Society-Division of Polymer Chemistry, and the American Association for the Advancement of Science.

                摘要:

                Ribozymes synthesize proteins in a highly regulated local environment to minimize side reactions caused by various competing species. In contrast, it is challenging to prepare synthetic polypeptides from the polymerization of N-carboxyanhydrides (NCAs) in the presence of water and impurities, which induces monomer degradations and chain terminations, respectively. Inspired by natural protein synthesis, we herein report the preparation of well-defined polypeptides in the presence of competing species, by using a water/dichloromethane biphasic system with macroinitiators anchored at the interface. The impurities are extracted into the aqueous phase in situ, and the localized macroinitiators allow for NCA polymerization at a rate which outpaces water-induced side reactions. Our polymerization strategy streamlines the process from amino acids towards high molecular weight polypeptides with low polydispersity by circumventing the tedious NCA purification and the demands for air-free conditions, enabling low-cost, large-scale production of polypeptides that has potential to change the paradigm of polypeptide-based biomaterials.


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